Cryptosporidium Reference Unit

Contact Us

Cryptosporidium Reference Unit
Public Health Wales Microbiology ABM
Singleton Hospital
Sgeti
Swansea
SA2 8QA    

Public Health Wales Microbiology ABM
DX 6070300
Swansea 90 SA

Tel:  01792 285341 (for results, consultation and advice during office hours)
Tel (out of hours): 01792 205666 (ask for on-call consultant microbiologist)
Fax:  01792 202320
Email: Swansea.crypto@wales.nhs.uk
Laboratory hours: 0845-1700 (Monday to Friday)

Staff

  • Consultant Clinical Scientist and Head of Unit, Professor Rachel Chalmers
  • Principal Clinical Scientists, Dr Kristin Elwin and Dr Guy Robinson
  • Consultant Medical Microbiologist, Dr Angharad Davies
  • Lead Biomedical Scientist, Heather Jones FIBMS
  • Biomedical Support Worker, Jonathan Goss
  • Research Scientist, Dr Gregorio Perez Cordon

Core Reference Services and Guide to Investigations

  • Differential diagnosis and confirmation of referred isolates of Cryptosporidium, 
  • Specialist testing for the detection of Cryptosporidium in immunocompromised patients at high risk 
  • Typing of referred isolates to support outbreak/cluster investigations and for epidemiological surveillance 
  • Evaluation of new laboratory methods for Cryptosporidium 
  • Provision of positive control and training materials 
  • Consultation advice on diagnostic methods and management of cryptosporidiosis 
  • Advice on the investigation, epidemiology, control and prevention of Cryptosporidium 
  • R & D activities in relation to our reference functions

Investigation

 

Samples required Tests performed

Turnaround time

(depends on complexity and current workload)

Confirmation

Stool

DNA (min 20µl) - by prior arrangement

Microscopy

PCR

2 working days

Specialist testing of immunocompromised patients at high risk from Cryptosporidium

Stool

(minimum 2 scoops)

Other specimen types as indicated

Microscopy (including immunofluorescence)

PCR-based tests

2 working days

 

PCR offered weekly

Typing of Cryptosporidium isolates (species/genotype) for epidemiological purposes and for the investigation of clusters/outbreaks

 

Further characterisation is at the discretion of the Unit Head or following consultation

 Cryptosporidium-positive stools (minimum 2 scoops, ideally without preservative but if formalin has been added, this should be noted on the form)

 

DNA (min 20µl) - by prior arrangement

 

 

 PCR-based tests

 5-10 working days (maybe reduced during outbreak investigations)

Typing from Cryptosporidium-positive water and environmental slides

   Please see our water and environmental services webpage for details

How to Obtain Services 

Use the Cryptosporidium Reference Unit form:

Sample transportation

  • Samples should be sent in packaging appropriate to current legislation, accompanied by a referral form (available to download above) detailing putative diagnosis, patient or sample details and relevant (clinical) history. 
  • Send either by Royal Mail, HAYSDX or other Courier Services
  • Cryptosporidium positive stools for typing may be batched prior to sending to us if this is more convenient for you. Please store faeces in a refrigerator 
  • Category 3 organisms: Requests for work on samples also known to contain organisms in ACDP Hazard Group 3 must be clearly marked.

Charges

Stool testing service is free of charge to all NHS Trust, Public Health England and Public Health Wales laboratories in England and Wales, to whom Cryptosporidium positive control stools for microscopy and enzyme immunoassays can be supplied on request,

Charges for investigations for other purposes are reviewed annually and can be supplied on request.

External links