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DIGEST – Typing Clostridioides difficile by WGS

Scientific Lead: Trefor Morris

Clinical Lead: Dr Harriet Hughes


Current typing by PCR Ribotype (developed in UK Anaerobe Reference Unit, Cardiff)

WGS typing much more discriminatory

Benefits – understanding epidemiology to inform action:

    • Around three quarters of individual cases were genetically unrelated at 2 snp’s  (similar to previous work)
    • Considerable diversity within ribotypes:  e.g. approximately 64% of RT 002 were unrelated

Toxin positive and negative